Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon

Assessing the availability of dietary micro-minerals is a major challenge in mineral nutrition fish species. The present article aims to describe systematic approach combining different methodologies assess zinc (Zn) Atlantic salmon (Salmo salar). Considering that several Zn chemical species can be an feed, it was hypothesised influenced by feed. Thus, this study, first protocol about how extract from feed and analyze them size exclusion chromatography-inductively coupled plasma mass spectroscopy (SEC-ICP-MS) method. Subsequently, vitro method developed evaluate solubility feeds. third describes study impact changing composition on uptake intestinal epithelial model using rainbow trout gut cell line (RTgutGC). Together, findings methods were compared with vivo examining apparent inorganic organic sources supplemented results showed found feeds efficiency source depends very much amino acid ligand used chelate Zn. had less correlation outcome study. Nevertheless, protocols described provided crucial information regarding its assessment